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FoodEnTwin Winter School 2020

Dear colleagues, we are glad to announce

FoodEnTwin Winter School 2020

Proteomics: From sample preparation to practical aspects

February 5th – 8th, 2020, 

University of Belgrade-Faculty of Chemistry, 

Belgrade, Serbia

 

The Winter school is dedicated to PhD students, a post-doctoral scientists, or a junior researchers, who would like to gain basic training in proteomics. 

The Proteomics Winter school will provide an excellent opportunity for researchers from different fields related to proteomics to learn and share information on research, theories and especially practical aspects of proteomics. 

It will provide a space to exchange ideas and knowledge, as well as to enable communication, networking and promote international collaborations. It offers students and young researchers the opportunity to acquire new knowledge and practical skills related to proteomics, discuss problems, and obtain advice from experts, and in that way intends to support them in their scientific work.

Topics

The Winter school offers interesting lectures, dealing with theoretical and experimental aspects in proteomics, as well as practical training, presented by established scientists and experts. 

Sample preparation

Mass spectrometry analysis and instrumentation

Bioinformatics

Guided analysis of own sample

Lecturers

Tanja Ćirković Veličković, Professor (University of Belgrade-Faculty of Chemistry, Serbia), 

Dragana Stanić-Vučinić, PhD, Research Advisor (University of Belgrade-Faculty of Chemistry, Serbia), 

Tatjana Parac-Vogt, Professor (Katholieke Universiteit Leuven, Belgium),

Andrea Urbani, Professor (University Cattolica del Sacro Cuore and Fondazione Santa Lucia – IRCCS, Rome, Italy; President of European Proteomics Association),

Paola Roncada, Professor (University ‘Magna Græcia’, Catanzaro, Italy),

Danijela Apostolović, PhD, Research Associate (Karolinska Institutet, Sweden), 

Katarina Smiljanić, PhD, Senior Research Associate (University of Belgrade-Faculty of Chemistry, CoE- Serbia), 

Svetlana Murzina, PhD, Research Associate (Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences (IB KarRC RAS), Russian Federation),

Jelena Radosavljević, Assistant Professor (University of Belgrade-Faculty of Chemistry, Serbia), 

Luka Mihajlović, PhD (Analyisis doo., Belgrade),

Tamara Vasović, PhD student (University of Belgrade-Faculty of Chemistry, Serbia)

General Information

Number of attendees without fees is limited to 50 (40 from Serbia and 10 from abroad) and all applicants must apply via procedure described below. Accommodation and financial support covering the travel are available for 10 junior researchers and PhD students. Accommodation will be provided for maximum 4 nights (as of February 4th) and travel costs will cover a maximal amount of 350 EUR per person. The priority will be given to junior researchers/PhD students applying from FoodEnTwin consortium. 

Number of applicants accepted for practical training sessions: 50

Number of applicants that will be accepted for theoretical sessions: 125

Registration fee: 

Category

Fee

FoodEnTwin grant holder – 10 participants

Free of charge plus accommodation and travel 

Attendance to theoretical sessions and practical training – 50 participants

Free of charge

Attendance to theoretical sessions – 125 participants

25 EUR

Application procedure:

1) Fill in and sign application form (can be downloaded here)

Required attachments: 

  1. CV and a list of publications. 

  2. Motivation letter  (max 1500 characters)

  3. Evidence of employment as junior researcher or PhD student (provided by institution administration /office)

Optional:

  1. Research proposal (max 1 A4 page) – only if you want to analyze your selected sample during the practical training

3) Send all documents together in one email to:

foodentwinwp2@chem.bg.ac.rs

Only complete applications will be considered for financial support.

Application deadline for grants and practical training: November 15th 2019.

logo-FoodEnTwin

FoodEnTwin Winter School 2020

Proteomics: From sample preparation to practical aspects

February 5th-8th, 2020 

University of Belgrade – Faculty of Chemistry,

Centre of Excellence for Molecular Food Sciences (CoE-MFS) 

Studentski trg 16, Belgrade, Serbia

Program

Day 1 – Wednesday, February 05, 2020

9:00-9:30

Opening of the Winter School of Proteomics (dr Dragana Stanić- Vučinić and Vice Dean UBFC)- 1st floor, Lecture room-SZS

9:30 – 12:30

Practical part: Sample preparation for isoelectric focusing on IPG strips (dr Vesna Jovanović, dr Marija Peruško) – 1st floor, laboratories 402 and 403

12:30 – 14:00

Lunch – Restaurant Teatroteka, Gospodar-Jevremova 19 street http://www.teatroteka.rs/

14:00 – 16:30

Theoretical part: 1st floor, Lecture room-SZS 

• 14:00 – 14:45 Introduction – MS and proteomics (prof. Tanja Ćirković Veličković)

• 14:45 – 15:30 Maldi TOF and ESI MS analyses (prof. Tanja Ćirković Veličković)

• 15:30 – 16:00 Isoelectric focusing of high resolution (dr Jelena Radosavljević)

16:00 – 16:30

Coffee break

16:30 – 18:00

Practical part: Isoelectric focusing of high resolution (IPG) (Sara Trifunović, Mirjana Radomirović)- 1st floor, Labs 402/403

Day 2 – Thursday, February 06, 2020

9:00 – 12:30

Theoretical part: 1st floor, Lecture room 

• 9:00-9:45 Sample preparation for MS (J. Radosavljević)

• 9:45-10:30 nLC-MS/MS Orbitrap analysis of peptides (Tamara Vasović, Mass spectrometry Specialist at UBFC)

• 10:30 – 11:00  Coffee break

• 11:00 – 11:45 MS applications (dr Danijela Apostolović)

• 11:45– 12:30  MS applications (dr Svetlana Murzina)

12:30 – 14:00

Lunch – Restaurant Teatroteka http://www.teatroteka.rs/

14:00 – 17:00

Practical part: 

14:00 – 17:00 2D PAGE follow up and nanoLC Orbitrap LTQ XL (Sara Trifunović and Tamara Vasović) 2nd floor lab 516

Day 3 – Friday, February 07, 2020

9:00 – 12:30

Theoretical part: 1st floor, Lecture room 

• 9:00-9:45 MS applications (Professor Andrea Urbani)

• 9:45-10:30 MS applications (Professor Paola Roncada)

• 10:30 – 11:00  Coffee break

• 11:00 – 11:45 MS of intact proteins and peptides (dr Dragana Stanić-Vučinić)

• 11:45 – 12:30 Artificial proteases based on metal complexes for proteomics application (prof. Tatjana Parac-Vogt)

12:30 – 14:00

Lunch – Restaurant Teatroteka http://www.teatroteka.rs/

14:00 – 17:00

Practical part: 

• 14:00 – 17:00 2D PAGE follow up/in gel trypsin digestion (Maja Krstić-Ristivojević, Mirjana Radomirović) –2nd floor, room 516

20:00

Gala Dinner – „Dva Jelena“ Skadarlija, Skadarska 32 street, http://www.dvajelena.rs/en

Day 4 – Saturday, February 08, 2020

9:30 – 12:30

Theoretical part/ Bioinformatics 1st floor, Lecture room 

• 9:30-10:15 MS applications “Single cell peptidomics” (dr Luka Mihajlović, Mass spectrometry and applicative solutions specialist at Analysis d.o.o.)

• 10:15 – 10:45  Coffee break

• 10:45 –  11:30 Data base search and protein identification (dr Katarina Smiljanić)

• 11:30 – 12:15 DeNovo peptide search, Multiround protein search and unrestricted PTM search (dr Katarina Smiljanić)   

12:30 – 14:00

Lunch – Restaurant Teatroteka

14:00 – 17:00

Bioinformatics: Theoretical and practical, basement, Computational lab. 241 

Protein identification with selected samples and PEAKS X platform (dr Katarina Smiljanić and Tamara Vasović)

17:00 – 17:15

Closure of the school (D. Stanić-Vučinić)

Lecturers

Tanja Ćirković Veličković, Professor (University of Belgrade-Faculty of Chemistry, Serbia), 

Dragana Stanić-Vučinić, PhD, Research Advisor (University of Belgrade-Faculty of Chemistry, Serbia), 

Tatjana Parac-Vogt, Professor (Katholieke Universiteit Leuven, Belgium),

Andrea Urbani, Professor (University Cattolica del Sacro Cuore and Fondazione Santa Lucia – IRCCS, Rome, Italy; President of European Proteomics Association),

Paola Roncada, Professor (University ‘Magna Græcia’, Catanzaro, Italy),

Danijela Apostolović, PhD, Research Associate (Karolinska Institutet, Sweden), 

Katarina Smiljanić, PhD, Senior Research Associate (University of Belgrade-Faculty of Chemistry, CoE- Serbia), 

Svetlana Murzina, PhD, Research Associate (Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences (IB KarRC RAS), Russian Federation),

Jelena Radosavljević, Assistant Professor (University of Belgrade-Faculty of Chemistry, Serbia), 

Luka Mihajlović, PhD (Analyisis doo., Belgrade),

Tamara Vasović, PhD student (University of Belgrade-Faculty of Chemistry, Serbia)

Demonstrators

Vesna Jovanović, PhD (University of Belgrade-Faculty of Chemistry, Serbia)

Marija Peruško, PhD (University of Belgrade-Faculty of Chemistry, Serbia)

Maja Krstić-Ristivojević, PhD student (University of Belgrade-Faculty of Chemistry, Serbia)

Sara Trifunović, PhD student (Mediterranean Institute for Life Sciences- MedILS, Split, Croatia),

Tamara Vasović, PhD student (University of Belgrade-Faculty of Chemistry, Serbia) and

Mirjana Radomirović, PhD student (University of Belgrade-Faculty of Chemistry, Serbia).

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